Result examples
Sequencing and fragment analysis in applied workflows
NANOPHOR 05 produces STR profiles, microsatellite profiles and sequencing electropherograms for applied genetic studies.
- STR profiling
- Microsatellite analysis
- Sanger sequencing
Methods
Fragment analysis measures length; sequencing determines the nucleotide sequence
NANOPHOR 05 is used both to read nucleotide sequences and to measure the length of fluorescently labelled DNA fragments.
Fragment analysis
STR profiles, microsatellite markers, repeat-length analysis, allelic ladders and size standards.
Sanger sequencing
Targeted DNA sequencing for species identification, locus analysis and variant confirmation.
Application areas
What the analyzer shows in applied studies
The galleries show allelic ladders, control samples, microsatellite-marker profiles and sequencing electropherograms.
Forensic science
STR-panel fragment analysis: allelic ladders, positive controls and profiles for identification studies.
- The gallery shows allelic ladders and positive controls for PowerPlex® Fusion 6C, VeriFiler™ Express and GlobalFiler™.
- The Human ID kit covers 31 STR loci; its ladder, control and sample profiles are shown.
- All displayed profiles were produced by capillary electrophoresis using NANOPHOR 05.
Medical research
Fragment analysis of repeat regions and microsatellite markers in UGT1A1, AR and HTT, together with an MSI panel.
- The UGT1A1 examples show TA-repeat profiles in the gene promoter region.
- Separate electropherograms show repeat analysis in AR and HTT.
- The MSI example shows a profile across a 15-marker microsatellite panel.
Livestock farming
Microsatellite genotyping examples for cattle, pigs, sheep and horses.
- Gene Profile Cattle covers 16 microsatellite loci.
- Gene Profile Pig+ covers 21 loci; Gene Profile Sheep covers 12 loci plus amelogenin.
- Gene Profile Horse is used for genotyping across 17 microsatellite loci.
Veterinary science
Targeted sequencing of the E locus in a study of genetically determined dog coat color.
- The example shows a sequencing electropherogram for the E locus.
- The separation used PDMA-6 polymer and 36 cm capillaries.
- The data were provided by the POISK veterinary laboratory in Saint Petersburg.
Fisheries and aquaculture
Fragment analysis of seven sturgeon STR loci using the GeneExpert Sturgeon kit.
- The gallery shows an allelic ladder and a sample profile.
- The GeneExpert Sturgeon kit uses seven STR loci.
- PCR products were separated by capillary electrophoresis using NANOPHOR 05.
Plant science
Microsatellite profiles of potato and grape samples for comparison and genetic certification.
- The potato example shows a profile across 12 microsatellite loci.
- The grape example shows a profile across nine microsatellite loci.
- Both examples were produced by fragment analysis using NANOPHOR 05.
Plant quarantine
Mitochondrial-DNA sequencing for species identification of Lymantria monacha.
- The example shows a mitochondrial-DNA sequencing electropherogram for Lymantria monacha.
- The separation used PDMA-6 polymer and 50 cm capillaries.
- The sequence was processed in Mutation Surveyor®.
Fundamental research
Sanger sequencing of the Glycyrrhiza inflata ITS region.
- The example shows an electropherogram for the Glycyrrhiza inflata ITS region.
- The separation used PDMA-6 polymer and 50 cm capillaries.
- The data were provided by the Komi Science Center of the Ural Branch of the Russian Academy of Sciences in Syktyvkar.
Method support
Discuss whether the method fits your analytical task
The SYNTOL team provides guidance on Sanger sequencing, fragment analysis, reagent selection and data analysis.