MetaGen reagent kit
The MetaGen reagent kit is intended for rapid nucleic-acid extraction from samples containing humic acids, including soil, silt and decayed material.
MetaGen reagent kit
The MetaGen reagent kit is intended for rapid extraction of nucleic acids from soil, silt, decayed material and other samples containing humic acids, which strongly inhibit enzymatic manipulations with nucleic acids such as PCR and restriction reactions.
Extraction does not require expensive specialized equipment or additional reagents. The kit does not contain volatile hazardous chemicals such as chloroform, benzene or phenol. It is suitable for difficult samples with high concentrations of inhibitors, including black soil and decayed material. According to the source article, extraction takes 60 minutes excluding sample-preparation time.
Applications
The kit can be used in laboratories and research institutes to extract nucleic acids from soil samples for subsequent PCR amplification, diagnostic real-time PCR with commercial test systems, and metagenomic studies.
Samples
Any material containing humic acids can be used when the isolated nucleic acid is intended for subsequent molecular manipulations.
| Target sample | Without homogenization / automatic homogenization | Manual homogenization |
|---|---|---|
| Soil | 250–300 mg | 750–900 mg |
| Silt | 200–250 mg | 600–750 mg |
| Decayed sample* | 150–200 mg | 450–600 mg |
*The homogenization stage is mandatory.
The kit is designed for 50 extractions.
Share this article
Save the link or send the material to colleagues.
More articles
Related materials and recent knowledge-base publications.
Detection of DNA of Hymenoscyphus fraxineus using the reagent kit “Hymenoscyphus fraxineus-RT”
Detection of DNA of Hymenoscyphus fraxineus Using the “Hymenoscyphus fraxineus-RT” Reagent Kit. Detection of DNA of Hymenoscyphus fraxineus Using the…
ReadDetection of DNA of Diaporthe caulivora using the reagent kit “Diaporthe caulivora-RT”
Detection of DNA of Diaporthe caulivora Using the “Diaporthe caulivora-RT” Reagent Kit. Detection of DNA of Diaporthe caulivora Using the “Diaporthe…
ReadDetection of DNA of Clavibacter nebraskensis using the reagent kit “Clavibacter nebraskensis-RT”
Detection of DNA of Clavibacter nebraskensis Using the “Clavibacter nebraskensis-RT” Reagent Kit. Detection of DNA of Clavibacter nebraskensis Using the…
Read