Estimate consumption
Consider the number of reactions, repeats, and the expected period of use.
Product amount
Choose a synthesis scale for the planned workload
The optical-density scale indicates the expected amount of product. A larger scale usually lowers the unit cost, but it should match the laboratory need.
Review the parameter
Choose in configurator
Submit request
Compare unit cost
The chart shows how the price per base per OD changes.
Do not confuse it with packages
Synthesis scale and the number of separate packages are configured independently.
Optical-density units
OD describes measured material but is not a fixed molecule count
Oligonucleotides absorb UV light at 260 nm. Conversion from absorbance to molar and mass concentration depends on composition, sequence, molecular weight, and modifications.
Absorbance is measured at 260 nm using a known optical path.
It is calculated for the specific sequence and base order.
Represents the number of molecules per solution volume.
Uses the molecular weight of the complete construct.
Understanding OD
Optical density is linked to solution absorbance
For a 1 mL solution and 1 cm optical path, A260 is used as a quantitative oligonucleotide measure.
Sequence matters
Purines, pyrimidines, and nearest-neighbor stacking change absorbance.
Modifications matter
Labels may contribute to molecular weight and optical properties.
OD is not directly nmol
Equal absorbance can represent different substance amounts for different sequences.
Passport resolves the conversion
Molar and mass concentrations are calculated for the supplied product.
Planning
Choose scale by working consumption, not price alone
Larger scales usually reduce unit cost but may create unnecessary stock and storage burden.
Reaction count
Include technical repeats and optimization reserve.
Working concentration
Convert concentration and reaction volume into total material.
Project duration
Long projects benefit from aliquots and fewer freeze–thaw cycles.
Package count
Separate packages and total OD are independent order parameters.
Practical questions
Before choosing the parameters
Why does unit price decrease with scale?
Fixed production operations are distributed across a larger amount of product.
Can OD be converted to nmol in advance?
Exact conversion requires the sequence extinction coefficient and molecular weight.
Does scale define the number of tubes?
No. Package count is configured separately.
Доступные фасовки
В расчёте используются только активные фасовки из справочника.
Как фасовка влияет на стоимость одной буквы
График показывает ориентировочную стоимость синтеза одной стандартной буквы в пересчёте на 1 ОЕ. При большей фасовке удельная цена снижается.
| Scale | Price per base per OD | Change vs. minimum scale |
|---|---|---|
| 1 OD | 35 ₽ | baseline |
| 2 OD | 17.5 ₽ | −50,0% |
| 5 OD | 10.6 ₽ | −69,7% |
| 10 OD | 7.7 ₽ | −78,0% |
| 20 OD | 5.7 ₽ | −83,7% |
| 50 OD | 4.26 ₽ | −87,8% |
| 100 OD | 3.49 ₽ | −90,0% |
| 200 OD | 2.91 ₽ | −91,7% |
| 500 OD | 2.51 ₽ | −92,8% |
| 1000 OD | 2.26 ₽ | −93,5% |
Расчёт основан на активных ценовых группах стандартных букв A/C/G/T/U и предназначен для сравнения фасовок. Итоговая стоимость rows зависит от длины, модификаций, очистки, контроля и формата.
Next step
Calculate the sequence at the required scale
Choose the scale and other parameters to recalculate the complete construct.