Start with the application
Routine and sensitive applications may require different purification levels.
Product purity
Choose purification for the oligonucleotide application
Purification determines how effectively the full-length product is separated from synthesis by-products. The choice depends on length, modifications, and assay requirements.
Review the parameter
Choose in configurator
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Consider the construct
Length and modifications affect the required separation of the target product.
Do not confuse it with QC
Purification obtains the target product, while quality control confirms selected characteristics.
Purification technology
Why sequence length and chemistry change purification requirements
Each synthesis cycle is highly efficient but not quantitative. Shortened products therefore accumulate with sequence length and must be separated from the full-length oligonucleotide.
Small losses at every cycle accumulate as the chain grows.
A cumulative-yield example before purification.
Longer sequences contain a larger fraction of short products.
The actual result depends on length, composition, modifications, and method.
Purpose
Separate the full-length product from n−1, n−2, and other impurities
Purification reduces shortened chains and reaction by-products without changing the ordered sequence.
Length
More synthesis cycles increase cumulative incomplete coupling.
Modifications
Labels and other groups may require removal of unreacted components.
Assay sensitivity
Short products may reduce specificity and sensitivity in demanding applications.
Required amount
Method selection balances purity with post-purification yield.
PAGE
Length-based separation in a denaturing polyacrylamide gel
Length markers identify the target band while denaturing conditions reduce secondary-structure effects.
Principle
Products are separated mainly by molecular weight and length, then the target band is isolated and desalted.
Conditions
The described route uses 10–20% gels and 7 M urea.
Indicative purity
Reported ranges are 95–99% below 25 bases and 85–90% above 30 bases.
Constraints
The route is intended for modest amounts and G-rich structures can be difficult to resolve.
HPLC
Hydrophobicity-based chromatographic separation
Reverse-phase HPLC separates DMT-containing full-length product from shorter chains.
Principle
The target fraction is collected, deprotected, desalted, and may be checked by analytical gel.
Scale
The method supports larger quantities of purified material.
Indicative purity
A range around 90–95% is described for reverse-phase HPLC.
Constraints
Resolution may decrease above 50 bases and for stable G-rich structures.
Practical questions
Before choosing the parameters
Is the most complex purification always best?
No. Match the method to application sensitivity, length, modifications, required yield, and available catalog options.
Does purification confirm molecular weight?
No. Purification separates products; molecular-weight confirmation belongs to quality control.
Why are G-rich sequences difficult?
They may form stable secondary structures that impair separation even under denaturing conditions.
Как выбрать очистку
На этой странице показываются только варианты, реально доступные в справочнике сайта.
PAGE + HPLC
Комбинированная очистка для конструкций, где важны длина и химическая чистота.
Когда применять
- Двойные модификации
- Ответственные диагностические задачи
Когда не выбирать
- Когда комбинации нет среди активных вариантов
Показывается только при наличии такого варианта в справочнике.
PAGE
Гель-очистка отделяет полноразмерный продукт от укороченных последовательностей.
Когда применять
- Oligonucleotides средней длины
- Строгие требования к полноразмерности
Когда не выбирать
- Когда нужна хроматографическая оценка чистоты
Выбор зависит от длины и конструкции олигонуклеотида.
HPLC
Хроматографическая очистка для модифицированных и меченых oligonucleotides.
Когда применять
- Fluorophores and quenchers
- Гидрофобные модификации
Когда не выбирать
- Когда выбранный вариант отсутствует в справочнике
Используйте только доступные варианты из справочника ниже.
No Purification
Активный вариант из текущего справочника.
Когда применять
- Когда этот вариант соответствует требованиям вашей задачи
Когда не выбирать
- Если требования эксперимента предполагают другой метод
Точные ограничения и условия уточняйте по описанию справочника и в редакторе заказа.
Доступные варианты очистки на сайте
Next step
Choose purification in the order configurator
Add a sequence, configure the parameters, and compare the indicative price of available options.