Synthesis for laboratory workflows
Oligonucleotide synthesis
Prepare standard and modified sequences, choose purification, quality control, synthesis scale, and delivery format. The system validates the parameters and calculates an indicative price before the request is submitted.
For lists, use Excel or CSV import and clipboard paste.
Production and quality control
What happens after the synthesis request is submitted
The oligonucleotide proceeds through automated phosphoramidite synthesis, deprotection, selected purification, desalting, quantitative measurement, and quality control. Product passport data accompany each item and support preparation of working solutions.
The stated production route targets at least 95% purity for standard and modified oligonucleotides.
Amount and concentration are measured spectrophotometrically with sequence-aware calculations.
Product is normally supplied lyophilized or dissolved in deionized Milli-Q water by agreement.
It records sequence, length, amount, concentration, molecular weight, and purification.
Production route
From solid-phase synthesis to a ready product
Each stage has a distinct role: assemble the chain, remove protecting groups, isolate the full-length product, remove salts, and quantify the material.
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Phosphoramidite synthesis
The sequence is assembled one residue at a time on an automated synthesizer. Length and chemistry affect the downstream purification challenge.
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Deprotection
The product is cleaved from the solid support and the protecting groups used during synthesis are removed.
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Purification
PAGE and/or reverse-phase HPLC separate the full-length oligonucleotide from short products and unreacted components.
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Desalting and measurement
Salts are removed, absorbance at 260 nm is measured, and concentration is calculated using the sequence extinction coefficient.
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Quality control
More than the amount is verified
The analytical route depends on the construct.
- Appearance and concentration
- Full-length product
- Chromatographic profile
- Modification functionality
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Product passport
Data for reproducible laboratory work
The passport links the ordered construct to the supplied material and supports accurate preparation of working solutions.
- Identification
- Amount
- Concentration
- Characteristics
Quality control
More than the amount is verified
The analytical route depends on the construct. Standard and labeled oligonucleotides require different confirmation methods.
Desalting and measurement
Salts are removed, absorbance at 260 nm is measured, and concentration is calculated using the sequence extinction coefficient.
Quality control
More than the amount is verified
The analytical route depends on the construct.
- Appearance and concentration The solution is inspected and the amount is determined from UV absorbance.
- Full-length product Gel electrophoresis with length markers helps assess the target band and shortened fragments.
- Chromatographic profile Analytical reverse-phase or ion-pair HPLC with a separation profile may be performed on request.
- Modification functionality Fluorescent labels, biotin, amino groups, and phosphate groups are confirmed with dedicated analytical procedures.
Product passport
Data for reproducible laboratory work
- Identification
- Amount
- Concentration
- Characteristics
Production route
From solid-phase synthesis to a ready product
Each stage has a distinct role: assemble the chain, remove protecting groups, isolate the full-length product, remove salts, and quantify the material.
1. Phosphoramidite synthesis
The sequence is assembled one residue at a time on an automated synthesizer. Length and chemistry affect the downstream purification challenge.
2. Deprotection
The product is cleaved from the solid support and the protecting groups used during synthesis are removed.
3. Purification
PAGE and/or reverse-phase HPLC separate the full-length oligonucleotide from short products and unreacted components.
4. Desalting and measurement
Salts are removed, absorbance at 260 nm is measured, and concentration is calculated using the sequence extinction coefficient.
Quality control
More than the amount is verified
The analytical route depends on the construct. Standard and labeled oligonucleotides require different confirmation methods.
Appearance and concentration
The solution is inspected and the amount is determined from UV absorbance.
Full-length product
Gel electrophoresis with length markers helps assess the target band and shortened fragments.
Chromatographic profile
Analytical reverse-phase or ion-pair HPLC with a separation profile may be performed on request.
Modification functionality
Fluorescent labels, biotin, amino groups, and phosphate groups are confirmed with dedicated analytical procedures.
Product passport
Data for reproducible laboratory work
The passport links the ordered construct to the supplied material and supports accurate preparation of working solutions.
Identification
Item name and the sequence written 5′→3′.
Amount
Optical-density units determined spectrophotometrically.
Concentration
Molar and mass concentrations calculated from sequence and molecular weight.
Characteristics
Length, molecular weight, modifications, and purification method.
Storage
Preserve quality after delivery
Storage depends on concentration, delivery format, and fluorescent labels. Aliquoting is useful for long-running work.
Short-term solution storage
At concentrations above 150 ng/µL (about 20 µM), aqueous solutions may be kept around 25 °C for approximately 1–2 weeks.
Long-term storage
For lower concentrations or longer use, −20 °C is recommended.
Freeze–thaw cycles
Repeated freezing and thawing should be avoided for diluted solutions.
Fluorescent labels
Labeled oligonucleotides should be protected from light and non-sterile contamination.
Practical questions
Before choosing the parameters
Is one OD a fixed number of nanomoles?
No. OD is based on absorbance at 260 nm, while conversion to moles and mass depends on length, composition, sequence, and modifications.
Why are purification and quality control separate?
Purification physically isolates the target product, while quality control confirms selected properties of the resulting material.
Can the product be supplied in solution?
Yes, when the format is available in the current catalog. Deionized Milli-Q water is normally used and the exact terms are recorded in the request.
Synthesis parameters
Build the product around the experimental task
Each parameter affects application, sample preparation, or result verification. Review the options before opening the configurator.
Construct
Production and delivery
How it works
A clear path from sequence to request
- 01Add sequences
Enter one item manually or import a list.
- 02Configure parameters
Choose scale, purification, quality control, format, and modifications.
- 03Validate and calculate
Resolve issues and review the indicative price.
- 04Submit the request
Send the prepared composition for final confirmation.
Tools
Choose the right preparation workflow
One sequence
A detailed form for precise construct and production configuration.
Multiple sequences
A list with row editing, bulk actions, import, and validation.
File import
Column mapping, preview, and batch preparation.
Before you start
What to consider
Q01Are purification and quality control the same?
No. Purification removes synthesis by-products, while quality control confirms selected characteristics of the final product.
Q02When should I choose a larger synthesis scale?
Base the choice on the required amount of material and the expected number of experiments. The scale page compares the indicative unit cost.
Q03Can I prepare a large list?
Yes. The configurator supports import, column mapping, preview validation, and bulk actions.
Q04Is the calculation a final price?
No. It is an indicative calculation based on the selected parameters. Final terms are confirmed after submission.
Ready to start?
Prepare your sequences and get an indicative calculation
The configurator keeps your parameters, shows issues, and helps prepare the request composition.