Define the verification goal
Identity, purity, and full length are confirmed by different methods.
Result verification
Quality control for the experimental requirements
Different methods confirm different properties of the final oligonucleotide. Choose quality control based on what must be verified before use.
Review the parameter
Choose in configurator
Submit request
Match the assay risk
The more sensitive the application, the more important it is to define the required verification level.
Review it with purification
Quality control does not replace purification; it complements the production route.
Analytical confirmation
Quality control answers a specific question about the final product
No single method simultaneously proves mass, purity, full length, and label functionality. Start with the property that is critical for the assay.
Spectrophotometry quantifies material using a calculated extinction coefficient.
Length markers help assess the target product and short fragments.
Analytical chromatography shows the major product and impurities.
Mass spectrometry compares measured and expected molecular weight.
Routine production control
What is checked for a standard oligonucleotide
Production measurements complement any selectable additional control.
Appearance
Solutions are inspected for clarity and visible particles.
Amount
UV absorbance at 260 nm is used to calculate OD and concentration.
Length and purity
Gel electrophoresis with length markers assesses the target product.
Separation profile
Analytical reverse-phase or ion-pair HPLC may be supplied on request.
Modified oligonucleotides
Label functionality requires dedicated confirmation
Correct mass alone may not prove that the functional group retains the required behavior.
Fluorescent label
The target band is visualized after electrophoresis under UV.
Biotin
Electrophoretic and colorimetric approaches are used.
Amino group
Reactivity may be checked through subsequent biotin conjugation.
Phosphate
An aliquot may be treated with alkaline phosphatase and compared electrophoretically.
Interpretation
Keep identity, purity, full length, and functionality separate
Quality control complements the selected purification route.
Identity
Expected molecular weight and correct modifications.
Purity
Proportion of target product relative to short chains and other impurities.
Full length
Conformity to the target sequence length.
Functionality
Ability of a label or linker to perform its experimental role.
Practical questions
Before choosing the parameters
Does mass spectrometry measure purity?
It primarily confirms mass and composition. Other methods are used for impurity profiling.
Is additional QC required after purification?
It depends on assay risk and which properties must be documented.
Why do modifications need separate tests?
A functional group may require confirmation of both presence and chemical or optical activity.
Как выбрать контроль качества
Показываются только активные виды контроля качества из справочника. Purification и контроль качества являются разными параметрами.
Mass Spectrometry
Подтверждает молекулярную массу и помогает выявить несоответствие состава.
Когда применять
- Модификации
- Зонды
- Высокая цена ошибки
Когда не выбирать
- Когда нужен только профиль чистоты
Подтверждает массу, но не заменяет оценку чистоты.
None
Активный вариант из текущего справочника.
Когда применять
- Когда этот вариант соответствует требованиям вашей задачи
Когда не выбирать
- Если требования эксперимента предполагают другой метод
Точные ограничения и условия уточняйте по описанию справочника и в редакторе заказа.
Available quality-control options
Next step
Add the required quality control to the sequence
The configurator shows available methods and includes them in the indicative calculation.