Preparation of genomic libraries for massively parallel sequencing using the SyntEra reagent kit
The SyntEra reagent kit is intended for preparation of genomic libraries for massively parallel sequencing. The article lists the kit composition and key workflow characteristics.
SyntEra: description and kit composition
The SyntEra reagent kit is intended for preparation of genomic libraries for massively parallel sequencing.
| Component | NGS-01-32 | NGS-01-96 |
|---|---|---|
| SyntEra SynMag beads | 10 mL | 2×10 mL |
| SyntEra 2X PCR | 1 mL | 3×1 mL |
| SyntEra TAG Buffer | 1 mL | 3×1 mL |
| SyntEra TAG | 40 µL | 110 µL |
| ddH2O | 2 mL | 4×2 mL |
Characteristics stated in the source material
- total genomic-library preparation time: about 1 hour;
- operator hands-on time: about 15 minutes;
- work with small amounts of input DNA, from 1 ng;
- compatibility with MPS sequencers: Nanofor SPS (SYNTOL, Russia); MiSeq, NextSeq 550/1000/2000 and NovaSeq 6000 (Illumina, USA); SURFSeq 5000, GenoLab M and FASTASeq 300 (GeneMind, China);
- developed and manufactured in Russia;
- delivery stated as 1 to 10 days depending on the region.
The source article also includes a MiSeq software screenshot illustrating sequencing quality for libraries prepared with the SyntEra kit.
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