Knowledge base
Fluorescent dyes
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Summary
This section describes fluorescent dyes used for oligonucleotide labeling, including carboxyfluorescein (FAM) and related fluorophores.
This section describes fluorescent dyes used for oligonucleotide labeling, including carboxyfluorescein (FAM) and related fluorophores. It summarizes key optical parameters such as absorption and emission maxima, molar extinction coefficient, quantum yield, photobleaching behavior and pH sensitivity, and indicates typical applications in DNA analysis, confocal microscopy and flow cytometry.
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