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Probes for real-time PCR

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Summary

Real-time PCR is a standard method for DNA and RNA analysis. Fluorescent probes are oligonucleotides carrying a fluorophore and a fluorescence quencher; their sequence is selected so that the probe anneals between the forward and reverse primers.

Real-time PCR is a standard method for DNA and RNA analysis. Fluorescent probes are oligonucleotides carrying a fluorophore and a fluorescence quencher; their sequence is selected so that the probe anneals between the forward and reverse primers. The section explains how probe design, dye selection and quencher placement affect background fluorescence, threshold cycle values and assay performance.

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