Animal genetic analysis, identification, and breeding traits
STR genotyping and defined genetic-marker analysis
The SYNTOL portfolio combines STR/microsatellite systems for genotyping and genetic passporting with assays for defined polymorphisms and markers. Results are expressed as genotype, STR profile, or marker result without automatically extending them into productivity or breeding-value claims.
The section combines STR genotyping and identification, genetic passporting and relatedness analysis where supported by the assay, and determination of defined SNPs, polymorphisms, and markers.
Laboratory tasks
- STR genotype cattle, sheep, pigs, horses, and sturgeon;
- generate genetic profiles and perform passporting where supported by the assay's intended use;
- determine defined polymorphisms in cattle;
- analyze Pig Screen markers;
- select a capillary-electrophoresis platform;
- open the current instruction;
- request fragment analysis and training.
Two analytical approaches
STR
- 01 DNA
- 02 Multiplex PCR
- 03 Capillary electrophoresis
- 04 STR profile / genotype / relatedness result
Within product scope
POLYMORPHISM MARKERS
- 01 Genomic DNA
- 02 Product-specific genotyping
- 03 qPCR with allele-specific probes
- 04 Genotype / marker result
qPCR applies to many cattle assays
Methods and technology support
Methods
qPCR is used for multiple polymorphism assays; STR systems and Pig Screen use multiplex PCR with capillary electrophoresis and fragment analysis.
Products for key stages
Gene Profile / GeneExpert: Cattle, Sheep, Pig+, Horse, and GeneExpert Sturgeon for STR/microsatellite genotyping.
Polymorphism and marker assays: CAPN1, CAST, GDF5, TG5, FANCI, BGL, BoLA-DRB3, bGH, CASB, and CVM/BLAD.
Pig Screen: a dedicated multiplex PCR panel followed by capillary electrophoresis.
"Meat Tenderness" reagent kit (CAPN1 C316G polymorphism)
"GenExpert-Sturgeon" reagent kit for genotyping sturgeon fish using multiplex PCR of seven nuclear microsatellite (STR) loci
Reagent kit "CVM-BLAD"
"Meat Tenderness" reagent kit (CAPN1 C4751T polymorphism)
"Meat Tenderness" reagent kit (CAPN1 Val530Ile polymorphism)
"Meat Tenderness" reagent kit (CAST C282G polymorphism)
"Meat Tenderness" reagent kit (CAST A2959G polymorphism)
Reagent kit for determination of polymorphism T586C gene GDF5 cattle
Reagent kit for determination of polymorphism C422T gene TG5 cattle
Reagent kit for detection of bovine brachyspina syndrome (BY)
Reagent kit for determination of polymorphism gene BGL
Reagent kit for determination of polymorphisms *11 and *23 gene BoLA-DRB3
Move from the task to concrete resources
Genotipirovanie pigs by 21-mu STR lokusu using the reagent kit “Gene Profile Pig +”
Open material↗Genotipirovaniya sheep by 12 STR loci and lokusu amelogenina using the reagent kit “Gene Profile Sheep”
Open material↗Genotipirovanie cattle by 16 microsatellite loci using the reagent kit “Gene Profile Cattle”
Open material↗SYNTOL equipment
NANOPHOR 05 genetic analyzer for molecular biology applications
Open equipment↗DNA Sequencing and Fragment Analysis
A comprehensive service package for DNA sample preparation, analysis and interpretation, with flexible configuration for the laboratory’s task.
Open service↗User Training
Practical user training for working with reagents, protocols and laboratory scenarios.
Open service↗Interpreting genetic results
A genetic marker, genotype, or STR profile is an analytical result and does not by itself guarantee phenotype, productivity, meat or milk quality, disease resistance, or breeding value.
Select an analysis
Primary actions: “Choose animal species,” “Choose STR or marker analysis,” “Select an assay,” “Open the instruction,” “Select a capillary-electrophoresis platform,” and “Request fragment analysis.”
Start a request