Phytosanitary molecular diagnostics
From crop and pathogen to the appropriate laboratory test
SYNTOL solutions for plant-pathogen detection combine plant-sample preparation, nucleic-acid extraction, and target-specific qPCR or RT-qPCR. Product selection is most useful when it begins with the crop and suspected pathogen.
The Phytoscreen portfolio includes nucleic-acid extraction products and assays for specific plant-pathogen DNA or RNA targets. This page helps laboratories select a product by sample type, crop, pathogen group, and analytical method.
Laboratory tasks
- prepare plant material for nucleic-acid extraction;
- select manual or automated extraction;
- process difficult plant samples and, for relevant products, soil or humic-acid-rich matrices;
- detect DNA from bacterial, fungal/oomycete, or nematode plant pathogens;
- detect viral or viroid RNA by RT-qPCR;
- select an assay by crop and target pathogen;
- verify compatibility of a specific assay with laboratory equipment.
Analytical sequence
Sample preparation
Homogenization and buffer treatment are used where required.
Nucleic-acid extraction
Depending on the sample, laboratories may use FitoSorb, FitoSorb-P, Phytoscreen-Express, CytoSorb, or documented automated formats.
Automation
Specific products document platforms including KingFisher Flex and TECAN.
Target detection
qPCR is used for DNA targets and RT-qPCR for RNA targets where specified by the kit.
The portfolio combines several nucleic-acid extraction options with a broad PH/PV assay series. It includes products for bacteria, fungi and oomycetes, nematodes, viruses and viroids across agricultural, ornamental and woody plant groups.
Key methods include plant-sample preparation and homogenization, DNA/RNA extraction, extraction automation, qPCR, and RT-qPCR.
Products for key stages
Nucleic-acid extraction options include FitoSorb, Phytoscreen-Express, CytoSorb, and automated variants. PH/PV assays are grouped by crop and pathogen; PH-001, PH-100m, PV-005, and PH-072 illustrate different task types without turning the page into a complete product list.
Reagent kit "FitoSorb"for isolation of nucleic acids from plant material (on magnetic particles)
"PhytoSorb-P" reagent kit for nucleic-acid isolation from plant material, with homogenization tubes (magnetic-particle method)
Reagent kit "FitoSorb-Automatic-48"for automated isolation of nucleic acids from plant material on robotic stations TECAN
Reagent kit "Phytoscreen-express"for isolation of nucleic acids plant material
«Phytoscreen-express-P» Reagent kit for isolation of nucleic acids from plant material
"CytoSorb" reagent kit for isolation of phytopathogen DNA/RNA from plant material, including complex samples
"MetaGen" reagent kit for nucleic acid isolation from soil, sludge, decayed material, and other samples containing humic acids
Extraction phosphate buffer (PBS)
Move from the task to concrete resources
“Phytoscreen” – technology for rapid molecular genetic diagnostics of plant diseases
Open material↗User Training
Practical user training for working with reagents, protocols and laboratory scenarios.
Open service↗Regulatory context and limitations
Sensitivity, specificity, and turnaround depend on the specific assay and protocol. Quarantine status, geographic distribution, and regulatory status of a pathogen are stated only from a dated authoritative source.
Select a test
Primary action: “Select a test by crop and pathogen.” Additional actions: “Select a nucleic-acid extraction method,” “Check equipment compatibility,” and “Request training.”
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