Choose the function
Start with the role the modification must perform in the experiment.
Functional construct
Add modifications for the method and application
Labels, quenchers, linkers, and other groups define oligonucleotide functionality. Review purpose, position, and compatibility before selection.
Review the parameter
Choose in configurator
Submit request
Check the position
Not every group is available at 5′, 3′, and internal positions.
Compare the price
The price depends on the selected modification and synthesis scale.
Functional groups
A modification should solve a specific experimental task
Position, incorporation route, purification, and sequence notation matter as much as the group name. The catalog below provides actual symbols and allowed positions.
Common for labels, phosphate, linkers, and protective groups.
May carry a label, quencher, extension blocker, or support-linked group.
Usually requires a modified residue or linker.
At least 95% purity is stated for purified fluorescent constructs.
Major classes
Choose the function, then the exact symbol
Modification classes change optical, chemical, or biological properties.
Fluorophores
Provide detection and may be terminal or internal when supported by the chemistry.
Quenchers
Suppress fluorophore emission in probes; the working range must overlap dye emission.
Biotin
Supports high-affinity avidin or streptavidin binding for immobilization and detection.
Amino linkers
Provide a reactive group for post-synthetic conjugation.
Spacers
Create distance or replace an unspecified sequence region.
Phosphorothioate bonds
Increase nuclease resistance for protected constructs.
Fluorescent labels
Incorporation route affects position, yield, and background
A dye may be incorporated during synthesis, through modified CPG, or post-synthetically through an amino linker.
Dye phosphoramidite
Introduces the label during solid-phase synthesis at an allowed position.
Modified CPG
Used for selected 3′ terminal dyes.
Post-synthetic conjugation
An activated dye reacts with an amino linker and requires additional purification.
Internal label
Often requires a modified dT linker and a dedicated sequence symbol.
Purification and control
Modified products must be separated from free label and unreacted chain
Fluorescent constructs use multi-stage separation followed by optical and size confirmation.
First stage
Gel filtration removes free dye and salts.
Second stage
HPLC or PAGE removes unreacted oligonucleotide.
Spectrophotometry
The spectrum confirms dye and nucleotide contributions.
Capillary electrophoresis
May be used for selected fluorescein-labeled products.
Practical questions
Before choosing the parameters
Why can natural symbol and symbol differ?
The public name describes the chemistry, while the technical symbol encodes position and sequence notation.
Can one dye be placed anywhere?
No. Allowed 5′, 3′, and internal positions depend on the exact chemistry.
Why are internal modifications more complex?
They require a modified residue or linker and may add synthesis and purification steps.
Список модификаций
Выберите группу, найдите модификацию по символу или названию и сравните ориентировочную стоимость для доступных фасовок.
Как читать каталог модификаций
Модификация важна не только сама по себе, но и тем, где она может стоять в последовательности. Перед заказом проверьте расположение, спектральные параметры и совместимость с очисткой.
Чаще используется для флуорофоров, биотина, фосфатных и других терминальных меток.
Обычно подходит для гасителей, блокирующих групп и терминальных модификаций зонда.
Подходит для dT/dC-модификаций и конструкций, где метка должна быть внутри цепи.
Для зондов сравнивайте λem красителя с диапазоном гашения, а не только названия.
Найдено модификаций: 30
применение: внутренняя позиция
| # | Симв. код | Name | Расположение | Экстинкция | Молек. масса | Price ? |
|---|---|---|---|---|---|---|
| Fluorophores | ||||||
| 1 |
dT-6FAM-C8N3FAM флуорофор |
Internal 6-FAM via Click Chemistry on a Long LinkerdT-6FAM-C8N3 Пример внутри: AC(dT-6FAM-C8N3)GTAC |
T | 30 | 971 |
от 481 ₽
|
| 2 |
T-Cy5Cy5 флуорофор |
Cy5 (Internal dT)T-Cy5 Пример внутри: AC(T-Cy5)GTAC |
T | 19 | 987 |
от 240 ₽
|
| 3 |
T-FAMFAM флуорофор |
6-Carboxyfluorescein (Internal dT)T-FAM Пример внутри: AC(T-FAM)GTAC |
T | 38 | 815 |
от 196 ₽
|
| 4 |
T-R6GR6G флуорофор |
Carboxyrhodamine 6G (Internal dT)T-R6G Пример внутри: AC(T-R6G)GTAC |
T | — | 896 |
от 240 ₽
|
| 5 |
T-ROXROX флуорофор |
6-Carboxy-X-Rhodamine (Internal dT)T-ROX Пример внутри: AC(T-ROX)GTAC |
T | — | — |
от 196 ₽
|
| 6 |
T-TAMRATAMRA флуорофор |
6-Carboxytetramethylrhodamine (Internal dT)T-TAMRA Пример внутри: AC(T-TAMRA)GTAC |
T | 32 | 869 |
от 240 ₽
|
| 7 |
T-Сy3Сy3 флуорофор |
Cy3 (Internal dT)T-Сy3 Пример внутри: AC(T-Сy3)GTAC |
T | 14 | 1 045 |
от 240 ₽
|
| 8 |
T-Сy5Сy5 флуорофор |
Cy5 (Internal dT)T-Сy5 Пример внутри: AC(T-Сy5)GTAC |
T | 19 | 987 |
от 240 ₽
|
| Quenchers | ||||||
| 9 |
T-BHQ1BHQ1 гаситель |
Black Hole Quencher 1 (Internal dT)<p><a class="float-start me-3" data-fancybox="filemanager" href="https://t.syntol-npk.ru/image/catalog/oligonucleotides/modifications/BHQ-2-dT.png"><img alt="" class… Пример внутри: AC(T-BHQ1)GTAC |
T | 30 | 955 |
от 240 ₽
|
| 10 |
T-BHQ2BHQ2 гаситель |
Black Hole Quencher 2 (Internal dT)T-BHQ2 Пример внутри: AC(T-BHQ2)GTAC |
T | 26 | 957 |
от 240 ₽
|
| Nucleobases | ||||||
| 11 |
II |
InosineI Пример 5′: I/ACGTACGT |
5′/X/3′ | 7 | 331 |
от 11 ₽
|
| 12 |
SH-dT |
5'-ThiothymidineSH-dT Пример внутри: AC(SH-dT)GTAC |
T | 9 | 578 |
от 51 ₽
|
| Affinity Tags | ||||||
| 13 |
Biotin-dTBiotin |
Biotin-dT (Glen Research)Biotin-dT Пример внутри: AC(Biotin-dT)GTAC |
T | 9 | 684 |
от 196 ₽
|
| Spacers | ||||||
| 14 |
dSpacer |
1',2'-DideoxyribosedSpacer Пример внутри: AC(dSpacer)GTAC |
X | — | 186 |
от 51 ₽
|
| 15 |
Spacer3 |
PropanediolSpacer3 Пример внутри: AC(Spacer3)GTAC |
X | — | 137 |
от 51 ₽
|
| 16 |
Spacer9 |
Triethylene GlycolSpacer9 Пример внутри: AC(Spacer9)GTAC |
X | — | 220 |
от 51 ₽
|
| 17 |
Spacer18 |
Hexaethylene GlycolSpacer18 Пример внутри: AC(Spacer18)GTAC |
X | — | 344 |
от 51 ₽
|
| Ungrouped | ||||||
| 18 |
2-AP |
2-Aminopurine2-AP Пример 5′: 2-AP/ACGTACGT |
5′/X/3′ | — | 313 |
от 108 ₽
|
| 19 |
4-Thio-dT4-Thio |
4-Thio-2'-deoxythymidine4-Thio-dT Пример 5′: 4-Thio-dT/ACGTACGT |
5′/T | — | 320 |
от 196 ₽
|
| 20 |
5-I-du |
5-Iodo-2'-deoxyuridine5-I-du Пример внутри: AC(5-I-du)GTAC |
U | — | 416 |
от 11 ₽
|
| 21 |
5-Me-dC |
5-Methylcytidine<p>5'-Dimethoxytrityl-N4-(O-levulinyl-6-oxyhexyl)-5-Methyl-2'-deoxyCytidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite</p>
<p>A branching monomer is required to construct comb-like oligonu… Пример внутри: AC(5-Me-dC)GTAC |
C | 5 | 303 |
от 24 ₽
|
| 22 |
5niInd |
5-Nitroindole5niInd Пример 5′: 5niInd/ACGTACGT |
5′/X/3′ | — | 332 |
от 108 ₽
|
| 23 |
6-thio-dG6-thio |
6-thio-dG-CE Phosphoramidite6-thio-dG Пример внутри: AC(6-thio-dG)GTAC |
G | — | 345 |
от 1 145 ₽
|
| 24 |
8-oxo-dG8-oxo |
8-oxo-deoxyGuanosine8-oxo-dG Пример внутри: AC(8-oxo-dG)GTAC |
G | 5 | 345 |
от 196 ₽
|
| 25 |
Alkyne-dTAlkyne |
Alkyne dT PhosphoramiditeAlkyne-dT Пример внутри: AC(Alkyne-dT)GTAC |
T | — | 437 |
от 196 ₽
|
| 26 |
Carboxy-dTCarboxy |
5'-Dimethoxytrityl-5-[3-methyl-acrylate]-2'-deoxyUridiCarboxy-dT Пример внутри: AC(Carboxy-dT)GTAC |
T | 9 | 360 |
от 51 ₽
|
| 27 |
di-AP |
Diaminopurinedi-AP Пример внутри: AC(di-AP)GTAC |
A | — | 344 |
от 51 ₽
|
| 28 |
iTiT |
3'-Inverted dTiT Пример внутри: AC(iT)GTAC |
T | — | 304.2 |
от 51 ₽
|
| 29 |
T-CholesterolCholesterol |
Cholesterol (Internal dT)T-Cholesterol Пример внутри: AC(T-Cholesterol)GTAC |
T | 9 | 888 |
от 240 ₽
|
| 30 |
T-NH2NH2 |
C6 Amino-dTT-NH2 Пример внутри: AC(T-NH2)GTAC |
T | 9 | 458 |
от 196 ₽
|
Next step
Build a modified sequence
Use the catalog as a reference and configure the final construct in the editor.