Functional construct

Add modifications for the method and application

Labels, quenchers, linkers, and other groups define oligonucleotide functionality. Review purpose, position, and compatibility before selection.

Configure and calculate About synthesis

Review the parameter Choose in configurator Submit request

Choose the function

Start with the role the modification must perform in the experiment.

Check the position

Not every group is available at 5′, 3′, and internal positions.

Compare the price

The price depends on the selected modification and synthesis scale.

Functional groups

A modification should solve a specific experimental task

Position, incorporation route, purification, and sequence notation matter as much as the group name. The catalog below provides actual symbols and allowed positions.

5′ terminal position

Common for labels, phosphate, linkers, and protective groups.

3′ terminal position

May carry a label, quencher, extension blocker, or support-linked group.

Internal internal position

Usually requires a modified residue or linker.

≥95% fluorescent-product purity

At least 95% purity is stated for purified fluorescent constructs.

Major classes

Choose the function, then the exact symbol

Modification classes change optical, chemical, or biological properties.

Fluorophores

Provide detection and may be terminal or internal when supported by the chemistry.

Quenchers

Suppress fluorophore emission in probes; the working range must overlap dye emission.

Biotin

Supports high-affinity avidin or streptavidin binding for immobilization and detection.

Amino linkers

Provide a reactive group for post-synthetic conjugation.

Spacers

Create distance or replace an unspecified sequence region.

Phosphorothioate bonds

Increase nuclease resistance for protected constructs.

Fluorescent labels

Incorporation route affects position, yield, and background

A dye may be incorporated during synthesis, through modified CPG, or post-synthetically through an amino linker.

Dye phosphoramidite

Introduces the label during solid-phase synthesis at an allowed position.

Modified CPG

Used for selected 3′ terminal dyes.

Post-synthetic conjugation

An activated dye reacts with an amino linker and requires additional purification.

Internal label

Often requires a modified dT linker and a dedicated sequence symbol.

Purification and control

Modified products must be separated from free label and unreacted chain

Fluorescent constructs use multi-stage separation followed by optical and size confirmation.

First stage

Gel filtration removes free dye and salts.

Second stage

HPLC or PAGE removes unreacted oligonucleotide.

Spectrophotometry

The spectrum confirms dye and nucleotide contributions.

Capillary electrophoresis

May be used for selected fluorescein-labeled products.

Practical questions

Before choosing the parameters

Why can natural symbol and symbol differ?

The public name describes the chemistry, while the technical symbol encodes position and sequence notation.

Can one dye be placed anywhere?

No. Allowed 5′, 3′, and internal positions depend on the exact chemistry.

Why are internal modifications more complex?

They require a modified residue or linker and may add synthesis and purification steps.

Список модификаций

Выберите группу, найдите модификацию по символу или названию и сравните ориентировочную стоимость для доступных фасовок.

All примененияПолный список 5′5′-конец 3′3′-конец ВнутриВнутренняя позиция ФлуорофорыСигнальные метки ГасителиQuencher-модификации Для зондовFluorophores and quenchers

Быстрый поиск: FAMфлуорофор BHQ1гаситель MGB3′-модификация зонда 2-APвнутренняя/концевая модификация

Как читать каталог модификаций

Модификация важна не только сама по себе, но и тем, где она может стоять в последовательности. Перед заказом проверьте расположение, спектральные параметры и совместимость с очисткой.

Probe selection

5′-конец39 вариантов

Чаще используется для флуорофоров, биотина, фосфатных и других терминальных меток.

3′-конец26 вариантов

Обычно подходит для гасителей, блокирующих групп и терминальных модификаций зонда.

Внутренняя позиция30 вариантов

Подходит для dT/dC-модификаций и конструкций, где метка должна быть внутри цепи.

Флуорофоры / гасители29 / 9

Для зондов сравнивайте λem красителя с диапазоном гашения, а не только названия.

Найдено модификаций: 9

применение: гасители

Reset filters

#	Симв. код	Name	Расположение	Экстинкция	Молек. масса	Price ?
Quenchers
1	`5'-RTQ1`RTQ1 гаситель	RTQ1 Amidite for 5' End5'-RTQ1 Пример 5′:`5'-RTQ1/ACGTACGT`	5′	8	658	—
2	`BHQ1`BHQ1 гаситель	Black Hole Quencher-1<p>With the growing popularity of red and near-infrared dyes, we are offering the Black Hole QuencherTM dyes (BHQs). BHQ dyes are robust dark quenchers that very nicely complement our existing product line. They ar… Пример 5′:`BHQ1/ACGTACGT`	5′/3′	8	569	от 98 ₽
3	`BHQ2`BHQ2 гаситель	Black Hole Quencher-2BHQ2 Пример 5′:`BHQ2/ACGTACGT`	5′/3′	8	571	от 98 ₽
4	`BHQ3`BHQ3 гаситель	Black Hole Quencher-3BHQ3 Пример 5′:`BHQ3/ACGTACGT`	5′/3′	13	612	от 98 ₽
5	`MGB`MGB гаситель	minor groove bindingMGB Пример 3′:`ACGTACGT/MGB`	3′	—	831	от 1 145 ₽
6	`RTQ1`RTQ1 гаситель	Real Time Quencher-1RTQ1 Пример 5′:`RTQ1/ACGTACGT`	5′/3′	8	660	от 98 ₽
7	`T-BHQ1`BHQ1 гаситель	Black Hole Quencher 1 (Internal dT)<p><a class="float-start me-3" data-fancybox="filemanager" href="https://t.syntol-npk.ru/image/catalog/oligonucleotides/modifications/BHQ-2-dT.png"><img alt="" class… Пример внутри:`AC(T-BHQ1)GTAC`	T	30	955	от 240 ₽
8	`T-BHQ2`BHQ2 гаситель	Black Hole Quencher 2 (Internal dT)T-BHQ2 Пример внутри:`AC(T-BHQ2)GTAC`	T	26	957	от 240 ₽
Ungrouped
9	`SH` гаситель	ThiolSH Пример 3′:`ACGTACGT/SH`	3′	—	322	от 51 ₽

Next step

Build a modified sequence

Use the catalog as a reference and configure the final construct in the editor.

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