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Detection of DNA of Bipolaris zeicola using the reagent kit “Bipolaris zeicola-RT”
Detection of DNA of Bipolaris zeicola Using the “Bipolaris zeicola-RT” Reagent Kit. Detection of DNA of Bipolaris zeicola Using the “Bipolaris zeicola-RT” Reagent Kit.
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Lineynye probes
linear probe, labeled by ends with different relative arrangement dyes: 1) Fluorophore — oligonucleotide — quencher; 2) quencher — oligonucleotide — Fluorophore.
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Fluorescent dyes
This section describes fluorescent dyes used for oligonucleotide labeling, including carboxyfluorescein (FAM) and related fluorophores.
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Probes for real-time PCR
Real-time PCR is a standard method for DNA and RNA analysis. Fluorescent probes are oligonucleotides carrying a fluorophore and a fluorescence quencher; their sequence is selected so that the probe anneals between the forward and reverse primers.
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Fluorescence quenchers
Dabsyl is used as a quencher for fluorophores with emission maxima from 450 to 550 nm and is commonly incorporated into oligonucleotide probes for quantitative real-time PCR.
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