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Detection of DNA of Bipolaris zeicola using the reagent kit “Bipolaris zeicola-RT”

Detection of DNA of Bipolaris zeicola Using the “Bipolaris zeicola-RT” Reagent Kit. Detection of DNA of Bipolaris zeicola Using the “Bipolaris zeicola-RT” Reagent Kit.

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Lineynye probes

linear probe, labeled by ends with different relative arrangement dyes: 1) Fluorophore — oligonucleotide — quencher; 2) quencher — oligonucleotide — Fluorophore.

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Fluorescent dyes

This section describes fluorescent dyes used for oligonucleotide labeling, including carboxyfluorescein (FAM) and related fluorophores.

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Probes for real-time PCR

Real-time PCR is a standard method for DNA and RNA analysis. Fluorescent probes are oligonucleotides carrying a fluorophore and a fluorescence quencher; their sequence is selected so that the probe anneals between the forward and reverse primers.

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Biotinylated oligonucleotides

Biotin has high affinity for avidin and streptavidin, so biotinylated oligonucleotides are used in hybridization, blotting, sequencing, genotyping and immobilization applications.

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Fluorescence quenchers

Dabsyl is used as a quencher for fluorophores with emission maxima from 450 to 550 nm and is commonly incorporated into oligonucleotide probes for quantitative real-time PCR.

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Fluorescently labeled oligonucleotides

SYNTOL uses synthesis and purification methods for fluorescently labeled oligonucleotides, including oligonucleotides carrying two dyes.

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Determining oligonucleotide concentration

Oligonucleotide concentration is determined from UV absorbance at 260 nm using the Beer–Lambert law and a molar extinction coefficient that depends on sequence and composition.

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Is oligonucleotide purification necessary?

Purification removes truncated synthesis products. The required purity depends on oligonucleotide length and the intended experiment; SYNTOL uses PAGE or reverse-phase HPLC.

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standard oligonucleotides

Name sequence 5'-3' length M13F(-20) gta-aaa-cga-cgg-cca-gtg 18 M13R(-27) gga-aac-agc-tat-gac-cat-g 19 M13F(-41) ggt-ttt-ccc-agc-cac-gac 18 M13R(-48) agc-gga-taa-caa-ttt-cac-ac 20 M13rev tca-cac-agg-aaa-cag-cta-tga-c 20 T7 taa-tac-gac-tca-cta-tag-gg 20 T3…

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Phosphorothioate oligonucleotides

Phosphorothioate oligonucleotides are used in antisense biotechnology as regulators of gene expression and are more resistant to cellular nucleases than unmodified oligonucleotides.

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Aminolinkery

Employees Syntol razrabotana technology synthesis and purification of oligonucleotides, containing aminolinker.

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