Practical materials for laboratories and research teams

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Differential diagnosis and detection RNA potato viruses using the reagent kit “Potato Virus M, Potato Virus Y, Potato Virus S-RT”

Detection of RNA of Potato Virus M, Potato Virus Y and Potato Virus S Using the “Potato Virus M, Potato Virus Y, Potato Virus S-RT” Reagent Kit. Detection of RNA of Potato Virus M, Potato Virus Y and Potato Virus S Using the “Potato Virus M, Potato Virus Y…

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MetaGen reagent kit

The MetaGen reagent kit is intended for rapid nucleic-acid extraction from samples containing humic acids, including soil, silt and decayed material.

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For analysis GMO

GMO Detect reagent kits manufactured by Syntol enable detection of plant-derived GMO lines registered in Russia and most GMOs registered worldwide.

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Reagent kits for tuberculosis diagnostics

Kits reagents “Amplitub” for rapid tuberculosis diagnostics, including with mnozhestvennoy and shirokoy lekarstvennoy resistance (MLU-TB, ShLU-TB).

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Lineynye probes

linear probe, labeled by ends with different relative arrangement dyes: 1) Fluorophore — oligonucleotide — quencher; 2) quencher — oligonucleotide — Fluorophore.

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Fluorescent dyes

This section describes fluorescent dyes used for oligonucleotide labeling, including carboxyfluorescein (FAM) and related fluorophores.

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Probes for real-time PCR

Real-time PCR is a standard method for DNA and RNA analysis. Fluorescent probes are oligonucleotides carrying a fluorophore and a fluorescence quencher; their sequence is selected so that the probe anneals between the forward and reverse primers.

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Biotinylated oligonucleotides

Biotin has high affinity for avidin and streptavidin, so biotinylated oligonucleotides are used in hybridization, blotting, sequencing, genotyping and immobilization applications.

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Fluorescence quenchers

Dabsyl is used as a quencher for fluorophores with emission maxima from 450 to 550 nm and is commonly incorporated into oligonucleotide probes for quantitative real-time PCR.

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Fluorescently labeled oligonucleotides

SYNTOL uses synthesis and purification methods for fluorescently labeled oligonucleotides, including oligonucleotides carrying two dyes.

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Determining oligonucleotide concentration

Oligonucleotide concentration is determined from UV absorbance at 260 nm using the Beer–Lambert law and a molar extinction coefficient that depends on sequence and composition.

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Is oligonucleotide purification necessary?

Purification removes truncated synthesis products. The required purity depends on oligonucleotide length and the intended experiment; SYNTOL uses PAGE or reverse-phase HPLC.

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